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실시간 PCR 기계

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ntscenter_JW_MS_K2WT001_S

실시간 PCR 기계

(Real time PCR system QuantStudio 3)

연구시설·장비 관리항목

시설장비등록번호	NFEC-2020-01-260155
모델명	QuantStudio 3
제작사	Thermo Fisher Scientific

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<p class="0" style="text-align:left;word-break:keep-all;mso-pagination:none;text-autospace:none;mso-padding-alt:0pt 0pt 0pt 0pt;"><center><span style="mso-fareast-font-family:나눔스퀘어; font-family:나눔스퀘어; font-size:14.0pt; font-weight:bold; ">실시간 </span><span style="mso-fareast-font-family:나눔스퀘어; mso-ascii-font-family:나눔스퀘어; mso-font-width:100%; letter-spacing:0pt; mso-text-raise:0pt; font-family:나눔스퀘어; font-size:14.0pt; font-weight:bold; ">PCR </span><span style="font-family:나눔스퀘어; font-size:14.0pt; font-weight:bold; ">기계</span></p></center>
<p class="0" style="text-align:left;word-break:keep-all;mso-pagination:none;text-autospace:none;mso-padding-alt:0pt 0pt 0pt 0pt;"><center><span style="font-family:나눔스퀘어; font-size:14.0pt; font-weight:bold; ">(Real time PCR system QuantStudio 3)</span></p></center>
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<p class="0" style="text-align:left;word-break:keep-all;mso-pagination:none;text-autospace:none;mso-padding-alt:0pt 0pt 0pt 0pt;"><span style="mso-fareast-font-family:나눔스퀘어; font-family:나눔스퀘어; font-size:12.0pt; font-weight:bold; ">연구시설</span><span style="mso-fareast-font-family:나눔스퀘어; mso-ascii-font-family:나눔스퀘어; mso-font-width:100%; letter-spacing:0pt; mso-text-raise:0pt; font-family:나눔스퀘어; font-size:12.0pt; font-weight:bold; ">·</span><span style="font-family:나눔스퀘어; font-size:12.0pt; font-weight:bold; ">장비 관리항목</span></p>
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    <td style="width:96.57pt;height:18.48pt;padding:1.41pt 5.10pt 1.41pt 5.10pt;border-top:solid #000000 0.28pt;border-left:solid #000000 0.28pt;border-bottom:solid #000000 0.28pt;border-right:solid #000000 0.28pt;" valign="middle"><p class="0" style="text-align:center;word-break:keep-all;mso-pagination:none;text-autospace:none;mso-padding-alt:0pt 0pt 0pt 0pt;"><span style="mso-fareast-font-family:나눔스퀘어; font-family:나눔스퀘어; font-weight:bold; ">시설장비등록번호</span></p></td>
    <td style="width:144.68pt;height:18.48pt;padding:1.41pt 5.10pt 1.41pt 5.10pt;border-top:solid #000000 0.28pt;border-left:solid #000000 0.28pt;border-bottom:solid #000000 0.28pt;border-right:solid #000000 0.28pt;" valign="middle"><p class="0" style="text-align:left;word-break:keep-all;mso-pagination:none;text-autospace:none;mso-padding-alt:0pt 0pt 0pt 0pt;"><span style="mso-fareast-font-family:나눔스퀘어; mso-ascii-font-family:나눔스퀘어; mso-font-width:100%; letter-spacing:0pt; mso-text-raise:0pt; font-family:나눔스퀘어; ">NFEC-2020-01-260155</span></p></td>
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    <td style="width:144.68pt;height:18.48pt;padding:1.41pt 5.10pt 1.41pt 5.10pt;border-top:solid #000000 0.28pt;border-left:solid #000000 0.28pt;border-bottom:solid #000000 0.28pt;border-right:solid #000000 0.28pt;" valign="middle"><p class="0" style="text-align:left;word-break:keep-all;mso-pagination:none;text-autospace:none;mso-padding-alt:0pt 0pt 0pt 0pt;"><span style="font-family:나눔스퀘어; ">QuantStudio 3</span></p></td>
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    <td style="width:144.68pt;height:18.48pt;padding:1.41pt 5.10pt 1.41pt 5.10pt;border-top:solid #000000 0.28pt;border-left:solid #000000 0.28pt;border-bottom:solid #000000 0.28pt;border-right:solid #000000 0.28pt;" valign="middle"><p class="0" style="text-align:left;word-break:keep-all;mso-pagination:none;text-autospace:none;mso-padding-alt:0pt 0pt 0pt 0pt;"><span style="font-family:나눔스퀘어; ">Thermo Fisher Scientific</span></p></td>
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연구시설·장비 소개

장비설명

sample내에 존재하는 target DNA/RNA의 양을 실시간으로 측정
5‘ Nuclease assay (TaqMan Probe), Double-stranded DNA binding dye (SYBR Green I) 응용
Bright white LED에서 나오는 빛을 각각의 well에 분사시켜 이로부터 방출된 형광을 감도가 뛰어난 CMOS camera로 검출

구성 및 성능

Sample capacity : 96
Reaction voulme : 0.1mL block : 10–30 μL0.2mL block : 10–100 μL
Excitation source : Bright white LED
Optical detection : 4 coupled filters
Excitation/detection range : 450-600nm/500-640nm
Heating/cooling method : Peltier
Max block Ramp rate : 6.5℃/sec
Average sample ramp rate : 3.66℃/sec
Temperature uniformity : 0.4℃
Temperature accuracy : 0.25℃
Dye compatibility : FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas Red

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<p class="0" style="text-align:left;word-break:keep-all;mso-pagination:none;text-autospace:none;mso-padding-alt:0pt 0pt 0pt 0pt;"><span style="mso-fareast-font-family:나눔스퀘어; font-family:나눔스퀘어; font-size:12.0pt; font-weight:bold; ">연구시설</span><span style="mso-fareast-font-family:나눔스퀘어; mso-ascii-font-family:나눔스퀘어; mso-font-width:100%; letter-spacing:0pt; mso-text-raise:0pt; font-family:나눔스퀘어; font-size:12.0pt; font-weight:bold; ">·</span><span style="font-family:나눔스퀘어; font-size:12.0pt; font-weight:bold; ">장비 소개</span></p>
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    <td style="width:79.59pt;height:82.48pt;padding:1.41pt 5.10pt 1.41pt 5.10pt;border-top:solid #000000 0.28pt;border-left:solid #000000 0.28pt;border-bottom:solid #000000 0.28pt;border-right:solid #000000 0.28pt;" valign="middle"><p class="0" style="text-align:center;word-break:keep-all;mso-pagination:none;text-autospace:none;mso-padding-alt:0pt 0pt 0pt 0pt;"><span style="mso-fareast-font-family:나눔스퀘어; font-family:나눔스퀘어; font-weight:bold; ">장비설명</span></p></td>
    <td style="width:396.55pt;height:82.48pt;padding:1.41pt 5.10pt 1.41pt 5.10pt;border-top:solid #000000 0.28pt;border-left:solid #000000 0.28pt;border-bottom:solid #000000 0.28pt;border-right:solid #000000 0.28pt;" valign="middle"><ul>
      <li><span style="mso-fareast-font-family:나눔스퀘어; mso-ascii-font-family:나눔스퀘어; mso-font-width:100%; letter-spacing:0pt; mso-text-raise:0pt; font-family:나눔스퀘어; ">sample</span><span style="mso-fareast-font-family:나눔스퀘어; font-family:나눔스퀘어; ">내에 존재하는 </span><span style="font-family:나눔스퀘어; ">target DNA/RNA</span><span style="font-family:나눔스퀘어; ">의 양을 실시간으로 측정</span></li>
      <li><span style="font-family:나눔스퀘어; ">5‘ Nuclease assay (TaqMan Probe), Double-stranded DNA binding dye (SYBR Green I) </span><span style="font-family:나눔스퀘어; ">응용</span></li>
      <li><span style="font-family:나눔스퀘어; ">Bright white LED</span><span style="font-family:나눔스퀘어; ">에서 나오는 빛을 각각의 </span><span style="font-family:나눔스퀘어; ">well</span><span style="font-family:나눔스퀘어; ">에 분사시켜 이로부터 방출된 형광을 감도가 뛰어난 </span><span style="font-family:나눔스퀘어; ">CMOS camera</span><span style="font-family:나눔스퀘어; ">로 검출</span></li>
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    <td style="width:79.59pt;height:194.48pt;padding:1.41pt 5.10pt 1.41pt 5.10pt;border-top:solid #000000 0.28pt;border-left:solid #000000 0.28pt;border-bottom:solid #000000 0.28pt;border-right:solid #000000 0.28pt;" valign="middle"><p class="0" style="text-align:center;word-break:keep-all;mso-pagination:none;text-autospace:none;mso-padding-alt:0pt 0pt 0pt 0pt;"><span style="font-family:나눔스퀘어; font-weight:bold; ">구성 및 성능</span></p></td>
    <td style="width:396.55pt;height:194.48pt;padding:1.41pt 5.10pt 1.41pt 5.10pt;border-top:solid #000000 0.28pt;border-left:solid #000000 0.28pt;border-bottom:solid #000000 0.28pt;border-right:solid #000000 0.28pt;" valign="middle"><ul>
      <li><span style="font-family:나눔스퀘어; ">Sample capacity : 96</span></li>
      <li><span style="font-family:나눔스퀘어; ">Reaction voulme : 0.1mL block : 10</span><span style="mso-fareast-font-family:나눔스퀘어; mso-hansi-font-family:나눔스퀘어; mso-font-width:100%; letter-spacing:0pt; mso-text-raise:0pt; font-family:나눔스퀘어; ">–</span><span style="font-family:나눔스퀘어; ">30 </span><span style="font-family:나눔스퀘어; ">μ</span><span style="font-family:나눔스퀘어; ">L0.2mL block : 10</span><span style="font-family:나눔스퀘어; ">–</span><span style="font-family:나눔스퀘어; ">100 </span><span style="font-family:나눔스퀘어; ">μ</span><span style="font-family:나눔스퀘어; ">L</span></li>
      <li><span style="font-family:나눔스퀘어; ">Excitation source : Bright white LED</span></li>
      <li><span style="font-family:나눔스퀘어; ">Optical detection : 4 coupled filters</span></li>
      <li><span style="font-family:나눔스퀘어; ">Excitation/detection range : 450-600nm/500-640nm</span></li>
      <li><span style="font-family:나눔스퀘어; ">Heating/cooling method : Peltier</span></li>
      <li><span style="font-family:나눔스퀘어; ">Max block Ramp rate : 6.5</span><span style="font-family:나눔스퀘어; ">℃</span><span style="font-family:나눔스퀘어; ">/sec</span></li>
      <li><span style="font-family:나눔스퀘어; ">Average sample ramp rate : 3.66</span><span style="font-family:나눔스퀘어; ">℃</span><span style="font-family:나눔스퀘어; ">/sec</span></li>
      <li><span style="font-family:나눔스퀘어; ">Temperature uniformity : 0.4</span><span style="font-family:나눔스퀘어; ">℃</span></li>
      <li><span style="font-family:나눔스퀘어; ">Temperature accuracy : 0.25</span><span style="font-family:나눔스퀘어; ">℃</span></li>
      <li><span style="font-family:나눔스퀘어; ">Dye compatibility : FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas Red</span></li>
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